Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
نویسندگان
چکیده
منابع مشابه
Transient gene expression in tobacco using Gibson assembly and the Gene Gun.
In order to target a single protein to multiple subcellular organelles, plants typically duplicate the relevant genes, and express each gene separately using complex regulatory strategies including differential promoters and/or signal sequences. Metabolic engineers and synthetic biologists interested in targeting enzymes to a particular organelle are faced with a challenge: For a protein that i...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2014
ISSN: 1940-087X
DOI: 10.3791/51234